SureSelect Target Enrichment Platform - Focus your NextGen on regions that Matters!
SureSelect target enrichment solutions allow you to target your research on the regions that matter most to you, whether analyzing the exome or focusing on a small targeted panel Agilent has a solution to meet your needs. Agilent’s SureSelect solutions for targeted DNA or RNA capture allow for you to increase sample throughput via multiplexing while achieving the sequencing depth required to accurately call SNPs, Indels and other mutations.
A complete and faster workflow
- Complete kits with reagents for gDNA prep, library prep and target enrichment
- Achieve your results faster with up to 96 indexes, the fastest hybridization time (only one 24hrs hyb) and a complete automation solution
Discover more with SureSelect All Exon Kits
- Comprehensive design covers the most current versions of primary genomic databases
- Available with or without UTRs, with improved uniformity for greater sample throughput
Your research, your way with flexible custom solutions
- Capture from <200kb to 34Mb+ for DNA and RNA
- Easily create designs with eArray, a free online tool, or use our design service
How it works
The SureSelect Target Enrichment workflow is solution-based system utilizing ultra-long – 120-mer – biotynilated cRNA baits – to capture regions of interest, enriching them out of a NGS genomic fragment library. Achieve your results faster with only 1x24hrs hybridization!
- Starting from gDNA, a shearing step produces small fragments.
- Prepare library with sequencer specific adaptors and indexes.
- Hybridize sample with biotinylated RNA library baits.
Agilent uses ultra long 120mer RNA baits for the highest specificity. Requires only 1 twenty four hour step. - Select targeted regions using magnetic streptavidin beads.
- Amplify and load on the sequencer.